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Effect of Pulsed Electric Field (PEF) and cryoconcentration (c) in aqueous extracts of maqui (Aristotelia chilensis (Mol.) Stuntz) and calafate (Berberis microphylla G.) on physicochemical properties, microbiological quality, and bioactive compounds
Indexado
WoS WOS:001354549400001
Scopus SCOPUS_ID:85208115259
DOI 10.1016/J.FBP.2024.10.021
Año 2024
Tipo artículo de investigación

Citas Totales

Autores Afiliación Chile

Instituciones Chile

% Participación
Internacional

Autores
Afiliación Extranjera

Instituciones
Extranjeras


Abstract



The objective of this study was to evaluate the effect of PEF treatment followed by cryoconcentration (c) in aqueous extracts of maqui (Aristotelia chilensis (Mol.) Stuntz) and calafate (Berberis microphylla G.) on their physicochemical properties, microbiological quality, and bioactive compounds. Extracts of maqui and calafate were obtained by aqueous extraction of water-soluble compounds, and then samples were treated by PEF (10 kV/cm, 70 Hz and a pulse width of 10 mu s) and pasteurization (90 degrees C/30 s); untreated samples were used as controls. The treated extracts were cryoconcentrated using centrifugation-filtration, and the cryoconcentration parameters included separation efficiency (EFF) and recovered solute (RS). Physicochemical properties were also evaluated, such as soluble solids (SS), titratable acidity (TTA), and viscosity (eta), as well as bioactive compound content: total polyphenols (TP), total anthocyanins (TA), total flavonoids (TF) and antioxidant capacity (AOXC). Microbiological quality was evaluated by the counts of the mesophilic aerobic microorganism (MAM) and molds and yeasts (M&Y). The results showed that the EFF of the solutes was higher than 95 % in maqui and 97 % in calafate. As expected, the cryoconcentration process increased TA, eta, and SS content. It also significantly increased (p<0.05) in TP, TF, TA, and AOXC content. The microbiological results showed that PEF treatment, together with cryoconcentration and refrigerated storage, maintained the microbiological quality of maqui cryoconcentrates for more than 50 days, whereas in calafate cryoconcentrates, it was maintained for similar to 20 days. Moreover, the physicochemical parameters and bioactive compounds of both cryoconcentrates remained stable during the refrigerated storage period. These results demonstrate that the combined use of PEF with cryconcentration seems to be a good option for microbiological and physiochemical stabilization of fruit juices with a high AOXC and for increasing the content of bioactive compounds.

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Disciplinas de Investigación



WOS
Biotechnology & Applied Microbiology
Engineering, Chemical
Food Science & Technology
Scopus
Sin Disciplinas
SciELO
Sin Disciplinas

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Publicaciones WoS (Ediciones: ISSHP, ISTP, AHCI, SSCI, SCI), Scopus, SciELO Chile.

Colaboración Institucional



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Autores - Afiliación



Ord. Autor Género Institución - País
1 Martin, Carla Vidal- San - Universidad del Bío Bío - Chile
1 Vidal - San Martín, Carla - Universidad del Bío Bío - Chile
2 BASTIAS-MONTES, JOSE MIGUEL Hombre Universidad del Bío Bío - Chile
3 Tamarit-Pino, Yanara - Universidad del Bío Bío - Chile
4 Reyes-Parra, Juan Esteban Hombre Universidad del Bío Bío - Chile
5 PEREZ-WON, MARIO OSVALDO Hombre Universidad del Bío Bío - Chile

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Financiamiento



Fuente
Universidad del Bío-Bío
National Agency for Research and Development (ANID)
Agencia Nacional de Investigación y Desarrollo
Agenția Națională pentru Cercetare și Dezvoltare

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Agradecimientos



Agradecimiento
The authors would like to thank the National Agency for Research and Development (ANID) for financial support through a National Ph.D. scholarship and the Universidad del Bio-Bio for its support in using infrastructure and equipment to develop this research.
The authors would like to thank the National Agency for Research and Development (ANID) for financial support through a National Ph.D. scholarship and the Universidad del B\u00EDo-B\u00EDo for its support in using infrastructure and equipment to develop this research.

Muestra la fuente de financiamiento declarada en la publicación.