Umami taste compounds are perceived in broilers through taste buds that detect peptides and amino acids, which can positively or negatively affect their feeding behavior. In this study, we evaluated the intake behavior for four essential amino acids (Lysine, Methionine, Threonine, and Tryptophan) in chickens. Sixty-four one-day-old male birds (Ross 308) were used. For 16 days during the early stage of the birds, two-choice preference tests were performed, in which 16 combinations composed of four amino acids in four concentrations (0.1 to 1.5%) diluted in water were evaluated, which were supplied in contrast to the delivery of water (a neutral compound) to a pair of birds in a pen for 4 h of administration after a prior 1 h fast. Amino acid solutions such as Threonine and Tryptophan tended to show less preference at the highest exposed concentrations (1.5%) concerning drinking water, which was confirmed in the case of Threonine when performing a sensory-motivated intake analysis (SMI). The opposite occurred with Lysine (1.5%), which numerically showed a higher preference ratified by SMI and acceptability analysis concerning water and other concentrations of the same amino acid, respectively. When palatability was measured with pecking cluster size, no significant differences across amino acid concentrations were observed, which is probably attributed to short recording periods and differences in solution intake behavior between chickens and previous experimental models such as rats. The results reinforce the notion that it is necessary to standardize feeding behavior tests in birds according to their feeding patterns and nutritional needs.