For the design of structures built with bamboo Guadua angustifolia as structural material it is necessary to know the characteristics values of its mechanical properties and elastics modulus. This article shows the experimental methodology used in the determination of characteristics values for bending, compression, shear and tensile parallel to fiber, torsion, compression perpendicular to fiber and elastics modulus for Guadua angustifolia. Tests were performed in samples taken from the bottom, middle and upper parts of culms of bamboos from three different areas of Colombia. Characteristic values of compression, tension and shear parallel to the fiber, bending, torsion and perpendicular compression strengths were found. Also the elastic longitudinal modulus and the circumferential modulus were determined.