Hematology and serum biochemistry values were determined for 31 healthy captive and free-ranging Culpeo foxes (Lycalopex culpaeus) sampled in central Chile between 2008 and 2012. The influences of sex, age, and origin (captive versus free-ranging foxes) on the blood parameters were evaluated. The blood values determined were generally comparable to commonly reported values for other wild canid species and the domestic dog. No differences attributable to sex were observed for any parameter. Juveniles had higher levels of alkaline phosphatase and phosphorus and lower values of mean corpuscular hemoglobin concentration, blood urea nitrogen, total protein, and globulin than adult foxes. Captive and free-ranging animals differed in glucose and albumin values. This is the first study on blood parameters of the Culpeo fox and represents a contribution for clinical evaluations of this carnivore in captivity as well as in the wild.