This study describes three new aeglid species, Aegla meloi, Aegla loyolai and Aegla lancinhas, all of them recorded from Parana. State, Brazil. Along with the description, we also present an analysis of their phylogenetic relationships to other species of Aegla. The conservation status using the IUCN Red List criteria was assessed for each new species. The new species have restricted distributions and can be found in altitudes higher than 700 m. The new species are phylogenetically close to other species present in Parana State and Ribeira do Iguape River basin, yet clearly differ morphologically and genetically from other known species of Aegla.