The in vitro susceptibilities of 24 worldwide Exserohilum isolates belonging to 10 species from human and environmental sources were determined for eight antifungal drugs. The strains were characterized by internal transcribed spacer (ITS) sequencing and amplified fragment length polymorphism fingerprinting. Posaconazole had the lowest geometric mean MIC (0.16 mu g/ml), followed by micafungin (0.21 mu g/ml), amphotericin B (0.24 mu g/ml), itraconazole (0.33 mu g/ml), voriconazole (0.8 mu g/ml), caspofungin (1.05 mu g/ml), isavuconazole (1.38 mu g/ml), and fluconazole (15.6 mu g/ml).