Background: Nosocomial infections are common adverse events associated with increased morbidity, mortality, and costs of patient care. Catheter-related bloodstream infections (CR-BSI) are nosocomial infections associated with higher medical costs. Aims: To evaluate CR-BSI associated costs in the Hospital Militar of Santiago, Chile, during year 2013. Methods: Comparative study between cases (CR-BSI) and matched controls using the Pan American Health Organization protocol. Variables were excess in length of stay (LOS), antimicrobial use according to daily defined doses (DDD), and total number of microbial cultures per hospitalization which were compared with non-parametric tests. Results: We included 10 cases and 10 matched controls. Mean LOS among cases was 40 days vs. 20.3 among controls (excess 20.3 days per event; p < 0.05). Antimicrobial consumption was higher among cases (DDD 36 vs. 10.5; p <0.05) and there was a trend to an increased number of bacterial cultures among cases (9 vs. 5; p = 0.057). The additional cost for the 10 subjects was 38 Chilean million pesos (USD 72,869) with a mean of 7,286 USD per event. Conclusions: During one year, CR-BSI generated an excess in LOS, antimicrobial consumption, and costs (7,286 USD per event of CR-BSI).