Although several reports on the variations of some radio observed ionospheric properties prior to the very large Chile earthquakes of 21-22 May 1960 have been published, no one up to now has reported on the variations of simultaneous E- and F-region characteristics observed at Concepcion (36.8 degrees S; 73.0 degrees W) using a ground based ionosonde. This paper analyses values of the NmE, NmEs, h'E, NmF2, h'F, M3000F2 and fmin. Possible solar and geomagnetic activity effects are first identified and then anomalies are calculated for all characteristics using reference values (15-day running medians +/- interquartile range). Occasions when anomalies are larger than an upper threshold and less than a lower threshold are discussed and compared, whenever possible, with other published studies. Further study is suggested to unambiguously associate some found possible Es-layer and M3000F2 anomalies with very strong earthquakes. (C) 2015 COSPAR. Published by Elsevier Ltd. All rights reserved.