To study the effect of micronutrients foliar applications in medicinal plants contain of Borago, Calendula and Thyme on yield and essence production, the investigations were performed in complete randomized block design with 3 replications at 2011 and 2012 in Shahrekord, Iran. Results showed that Fe2+, Cu2+, Zn2+ and Mn2+ applications on flavonoids, phenols, carotenoids, essence percentage, fresh/dry flower matter, number of flowers, and root/shoot dry/fresh matter in Borago officinalis and Calendula officinalis L.; carvacrol and thymol percentage, essence percentage, number of stem, height, and root/shoot dry/fresh matter in Thymus vulgaris L., the combinations of 400ppm of Fe, Cu,Zn and Mn (Fe3Cu3Zn3Mn3) produced the greatest amounts in most of measured characters. The most of phenols, carotenoids and flavonoids in Calendula and Borago; thymol and carvacrol in Thyme; weight of dry flower and number of flower per plant in two seasons were produced by Fe3Cu3Zn3Mn3 treatment.