Valley filters are crucial to any device exploiting the valley degree of freedom. By using an atomistic model, we analyze the mechanism leading to the valley filtering produced by a line-defect in graphene and show how it can be inverted by external means. Thanks to a mode decomposition applied to a tight-binding model we can resolve the different transport channels in k-space while keeping a simple but accurate description of the band structure, both close and further away from the Dirac point. This allows the understanding of a destructive interference effect, specifically a Fano resonance or antiresonance located on the p-side of the Dirac point leading to a reduced conductance. We show that in the neighborhood of this feature the valley filtering can be reversed by changing the occupations with a gate voltage, the mechanism is explained in terms of a valley-dependent Fano resonance splitting. Our results open the door for enhanced control of valley transport in graphene-based devices.