One beige-pigmented, Gram-staining-negative, rod-shaped bacterium, strain E3/2(T), was isolated from a zebrafish, Daniorerio. Phylogenetic analysis based on nearly full-length 16S rRNA gene sequences showed that the isolate shared 97.7% 16S rRNA gene sequence similarity to the species Pseudoduganella violaceinigra and between 97.4 to 97.0% to some species of the genera Duganella and Massilia, including Duganella radicis, Duganella phyllosphaerae, Massilia dura, Massilia lutea, Duganella sacchari, Duganella zoogloeoides, Massilia albidiflava and Massilia umbonata. Sequence similarities to all other species were below 97 %. The main cellular fatty acids of the strain were summed feature 3 fatty acids (C-16 : 1 omega 7c/iso-C-15 : 0 2-OH), C-10 : 0 3-OH, C-16 : 0 and C-12 : 0. The polyamine pattern of strain E3/2(T) contained predominantly putrescine and 2-hydroxyputrescine. The major quinone was ubiquinone Q-8. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Based on phylogenetic, chemotaxonomic, genomic and phenotypic analyses we propose a novel species of the genus Pseudoduganella named Pseudoduganella danionis sp. nov., with strain E3/2(T) (=LMG 29678(T) = CCM 8698(T)) as the type strain.