The sea otter (Lontra felina) lives along the rocky coastline exposed southeast Pacific between Peru (6 degrees S), Cape Horn, Chile (56 degrees S) and States island, Argentina. This native carnivore is classified as Vulnerable in the Red Book of Terrestrial Vertebrates of Chile. Between September and November 2004 we assessed their diet through fecal analysis and records of food remnants. We used a quantitative overlap index to test for coincidental similarity between the 2 analyses, and also we applied a survey to fisherfolks at Caleta El Manzano de Pucatrihue, southern Chile, to learn about the perception they have of L. felina impact on local fishing. Based on an analysis of 35 faeces the diet was represented by 60.8% of crustaceans, 32.4% of fishes and 6.7% of shellfishes presences. Of 150 food remnants the prey corresponds to 55.3% of crustaceans, 44.0% of molluscs and 0.7% of birds. The two analyses carried out had a slow similarity (= 0.35 +/- 0.22). L. felina preys on resources exploited by artisanal fishing, but fishing gears are not damaged by this marine mammal. A 66.7% of the fishermen said that otters not affect their fishing, while 30.0% said the opposite; holding as cause the consumption by L. felina on the same resources exploited by them (e.g., Concholepas concholepas). Although this mammal does not cause significant damage to local fisheries still exists at present, however, illegal hunting of this otter in the study area. The reasons for this action ranging from the sale of their skin until his killing by a simple recreation.