How is the eastern Antioquia 's Rural population distributed throughout the region? What effect does this distribution have for the region local development? Those are the questions for this article. A comparative and interpretative case study was used for qualitative methodology to answer the question above. Documental revision and semi-structured interviews were techniques used during research. This article is composed in three parts: the first part is a basic presentation of some theories that contribute to the issues. The second part is comprised of result and third part includes conclusions. The result indicates that there is a significant rural population (47%) with an uneven of property and rural rent (Gini of 0.50) and different lifestyle and consuming habits. This leads to the conclusion that the infrastructure works and rural rent are creating region of Antioquia zones of spatial segregation inside the Eastern region of Antioquia.