Background: Cardiovascular diseases (CVDs) are the first cause of death worldwide. Latin American countries including Chile has experience an increment in the prevalence of risk factors for CVDs. Aim: To estimate the prevalence of cardiovascular risk factors in workers from Universidad Austral de Chile and to evaluate its relation with age and sex. Subjects and methods: This was a cross-sectional study conducted in 2012 in 258 participants (55% males). Lifestyle and socio-economic questionnaire were applied and anthropometric (body mass index and waist circumference) and metabolic markers (glucose, lipid profile, and blood pressure) were measured in all participants. Results: Overall, 31.8% were obese, 38.9% had central obesity, 31.6% had high total cholesterol, 45.2% had lower HDL cholesterol, 34.4% had higher triglycerides, 7.4% had diabetes, 29.6% had hypertension, 35.6% had metabolic syndrome and 23.6% were physically inactive. Conclusions: The study population shows a high prevalence of cardiovascular risk factors. These cardiovascular risk factors increase with age, however this increase did not differ by sex.