Effects of plantation forestry on biodiversity are controversially discussed in literature. While some authors stress positive effects, others tend to attribute a largely negative influence to plantations. One important factor steering the influence on biodiversity are management practices. A second important factor is the environmental matrix. Chile offers the option to analyse both factors jointly. The coastal range of central Chile has experienced rapid and widespread replacement of native Nothofagus spp. forests in favour of Pinus radiata plantations. Here, native forests remain limited to small patches surrounded by an environmental matrix of plantations. Management is rather intensive and not designed to maintain biodiversity. While in the coastal range of central Chile the transformation from native forests to non-native tree plantations has almost come to an end, spatial extension of P. contorta and P. ponderosa plantations has just recently begun in Chilean Patagonia. While the management is similar to central Chile, plantations rather exist as small patches surrounded by an environmental matrix of native plant formations (e.g. Nothofagus spp. forests and Nothofagus spp. scrublands). In the framework of this work, effects of the two diametric land usages on biodiversity are assessed and compared. Biodiversity is assessed at the alpha-, beta- and gamma-scale. At the alpha-scale, biodiversity impacts are inferred statistically, using one-way ANOVA and Tukey's PostHoc test. Biodiversity of plants at both sites is significantly reduced in plantations when compared to native forests or scrublands. Plantation forestry lowers alpha-biodiversity and does not provide additional habitats for specialists. At the beta-scale, weak edge effects due to the presence of native forests are observed. In total, plantation forestry tends to promote plant invasions and impairs the survival of endemics. At the gamma-scale, plant species communities where predominantly native and endemic in forests, predominantly introduced in plantations. Positive effects of the more native environmental matrix in Patagonia are not found to be stronger than in central Chile, therefore it is concluded that management imposes a much stronger influence. Results show, that the biodiversity impacts in Central Chile are transferable to Chilean Patagonia, where plantation forestry is increasingly established. (C) 2017 Karlsruhe Institute of Technology. Published by Elsevier B.V.