A new probe (E)-7-(diethylamino)-3-(3-(4-fluorophenyl)-3-oxoprop-1-en-1-yl)-1-methylquinolin-2(1H)-one (DQCh) was synthesized and structurally characterized by nuclear magnetic resonance spectroscopy and HRMS. DQCh was photophysically characterized by employing UV-vis, fluorescence, and time-resolved fluorescence spectroscopy in combination with computational methods. This probe was studied as a turn-off fluorescent probe based on a Michael addition mechanism for sensing bisulfite (HSO3-), which is favored in the presence of an aqueous micellar solution of cetyltrimethylammonium bromide. The probe exhibited an effective and high selectivity toward bisulfite over other interfering anions with a detection limit of 0.7 μmol L-1. Moreover, probe DQCh showed great potential for its practical application in the detection of bisulfite in real samples of white wine.