Proanthocyanidins (PAs) extracted from grapes have several bioactivities, which have attracted attention for pharmacological uses. However, PA instability in the digestive tract and low intestinal absorption limits their use for oral administration. An alternative to overcome these challenges is encapsulation. In this study, PAs extracted from grape seed were encapsulated in polylactic acid (PLA), polylactic acid with polyethylene glycol (PLA/PEG) and chitosan succinyl lauryl (LSC), and the transport particles and cytotoxicity were evaluated. PLA and PLA/PEG particles had encapsulation close to 80%EE, were spherical in shape, had sizes in the range of 300-400nm and had a negative zeta potential. LSC particles had an encapsulation of 40%EE, semi-spherical morphology and micrometric size. Particles based on PLA/PEG and LSC significantly increased the apparent permeability coefficient (P-app) of PAs in Caco-2 cells compared to the unencapsulated extract, indicating that these types of particles could be used as a delivery system for PA grape seed, allowing it to increase its bioavailability.