This study monitored the conditioning of rainbow trout (Oncorhynchus mykiss) broodstock in a closed culture system at 3000 m above sea level (m.a.s.l.) to evaluate the feasibility of this culture method and assess the first spawning of the species under these conditions as an alternative production route for the communities present in the foothills of Cordillera de los Andes, northern Chile. A total of 120 specimens with an initial weight of 170 g were used. They were maintained in a recirculation aquaculture system (RAS) composed of protected circular tanks, decanters, a biofilter, and oxygenation equipment. Fish growth was monitored through parameters such as specific growth rate (SGR), feed conversion ratio (FCR) and percent weight growth (PWG), parameters that were maintained within those reported by other authors for this species. The first successful spawning occurred after 12 months of broodstock conditioning, yielding 8570 eggs, of which 6569 hatched successfully. The success of this conditioning and spawning not only broadens the understanding of adaptability to high-altitude environments, but also provides evidence for the potential diversification of productive activities in foothill areas through aquaculture.