Introduction: This study aimed to quantify and analyse scientific activity in Comparative Psychology and Animal Behaviour across the 19 Spanish-speaking countries in Latin America. This was achieved by examining articles published in journals indexed in the Web of Science Core Collection from 1975 to 2024 by authors affiliated with institutions in these countries. Method: A bibliometric analysis was conducted using 26 journals specialised in Comparative Psychology and Animal Behaviour, covering aspects such as historical production, authorship analysis, manuscripts, citations, countries, affiliations, and funding sources. The most relevant keywords, thematic mapping, thematic trends, and most frequently studied species were also identified. Results: A total of 1,971 articles were found. Most of the research output from Spanish-speaking countries in Latin America was concentrated in Mexico (35.1%), Argentina (21.6%), and Panama (12.8%). The contributions were mainly from authors associated with the Universidad Nacional Autónoma de México (16%), the Smithsonian Tropical Research Institute (14%), and the Universidad de Buenos Aires (8%). Additionally, it was identified that animal welfare and sexual selection are central themes in the field. Further indicators are presented to reflect the status of comparative psychology and animal behaviour research in this region. Conclusion: The primary limitations and gaps in the region are discussed, highlighting areas for improvement in the field.