Background and objective: Padel has experienced significant growth. It requires repetitive and intense motor gestures (MG), which are associated with musculoskeletal injuries (MSI). The objective was to evaluate shoulder and elbow postures during the tray serve MG in amateur padel players from Talca, Chile. Methodology: For this observational and cross-sectional study, 30 padel players from Talca, Chile were recruited. They were grouped into those with a history of MSI (wMSI, n = 14, age [95% CI] = 32.9 [29.5-36.4] years) and without a history of MSI (woMSI, n = 16, age [95% CI] = 29.3 [26.7-31.8] years). Angular positions in a sagittal plane of the shoulder and elbow were analyzed for the MG of the serve during a regular game in an official championship. Photogrammetry (Kinovea®) was used for the initial (beginning of the gesture), mid (maximum extension to prepare the stroke), and final (ball impact) postures. Results: For the shoulder, the initial position is similar in both groups (∼21° flexion), while the mid position is significantly more extended in the woMSI athletes (∼55 vs. ∼45°; P=.002), and this remains in the final phase (∼38 vs. ∼32°). For the elbow, the postures are similar (∼120°). The woMSI athletes achieve greater relative extension at the moment of ball impact (∼141 vs. ∼130°). Conclusions: The beginning of the MG of the serve shows similar angular postures among all padel players. However, the woMSI athletes exhibit a posture with greater shoulder and elbow extension during the mid and final phases.