Background: The blueberry (Vaccinium spp.) is a fruit commercially known for its high quality and health benefits, particularly for its bioactive antioxidant compounds, which are important in the medical field. However, factors such as genotype, stage of fruit ripening and environmental conditions impact the biosynthesis of bioactive compounds in the berry, as well as their yield and cultivation costs. In Mexico, particularly in the state of Sinaloa, extreme climatic conditions limit the cultivation of blueberry and highlight the need for the development of new varieties with low chilling requirements and tolerance to drought conditions. Results: Through the comb ined use of somatic organogenesis and in vitro polyploidization, genetic variability was promoted in the commercial blueberry plant variety "Biloxi". To achieve this purpose, blueberry microcuttings were treated with colchicine (0.02%) for six hours for 2, 4, 6 and 8 consecutive days and induced to form shoots stomatal density and 9 lin in vitro with Zeatin (1 mg L-1). Out of 304 generated plants, 36 showed lower es showed higher stomatal density. Likewise, 5 and 49 lines presented lower and larger stomatal sizes, r compared to the control treatment. Ploidy analysis using flow cytometry showed the successful genera-tion of four octoploid blue berry plants. Conclusions: This work succes that presented histological, bi house conditions for fruit qua How to cite: Heraldez AA, Pe & ntilde;uelas SV, Jarpa-Tauler G, et al. Octoploid blueberry development for drought tolerance: A combi Electron J Biotechnol 2025;75 (c) 2025 The Author(s). Published by Elsevier Inc. on behalf of Pontificia Universidad Cat & oacute;lica de Valpar-a & iacute;so. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). esp ectively. In 9 lines, a higher chlorophyll content was found (10% to 200%) sfully generated new octoploid blueberry plants. Currently, all the lines ochemical and/or genetic modifications are being evaluated under green-lity and drought tolerance. ned approach of in vitro polyploidization and somatic organogenesis. . https://doi.org/10.1016/j.ejbt.2025.01.004.