Mental Health Literacy influences psychological help seeking and adherence to mental health treatments. Despite the discouraging mental health indicators, reliable measurement tools have not been tested in the university population of southern Chile. The present study evaluated the psychometric properties of the adapted version of the Mental Health Knowledge Questionnaire in university students. The scale was translated and adapted, expert judges evaluated its content validity, and its psychometric properties were explored in 251 university students in southern Chile. The adapted scale is valid, with a unifactorial structure with excellent reliability indicators, and converges with the intention of seeking professional help. This instrument represents a contribution in the measurement of this construct and in its theoretical understanding in the local population.