Direct ink writing (DIW) is an attractive, extrusion-based, additive manufacturing method for fabricating scaffold structures with controlled porosity using custom composite inks. Polycaprolactone-bioactive glass (PCL-BG) inks have gained attention for bone applications, but optimizing the formulation and fabrication of PCL-BG-based inks for improved printability and desired mechano-biological properties remains a challenge. This study employs a two-step design to systematically evaluate the effect of three factors in terms of PCL-BG composite printability and mechano-biological properties: ink preparation (acetone or dichloromethane (DCM) as the solvent, and mechanical compounding), the extrusion temperature (90 degrees C, 110 degrees C, and 130 degrees C), and the BG content (0%, 10%, and 20% BG). Pure PCL was used as the control. Rheological, calorimetric, and thermo-gravimetric analyses were conducted before printing. Cylindrical scaffolds and solid wells were printed to evaluate the printability, mechanical properties, and cytocompatibility. The scaffold porosity and pore size were carefully examined. Mechanical tests demonstrated that composite formulations with added BG and higher printing temperatures increased the elastic modulus and yield strength. However, PCL-DCM-BG combinations exhibited increased brittleness with higher BG content. Despite concerns about the toxic solvent DCM, the cytocompatibility was comparable to pure PCL for all ink preparation methods. The results suggest that the interaction between the ink preparation solvent, the BG content, and the printing temperature is critical for material design and fabrication planning in bone tissue engineering applications, providing insights into optimizing PCL-BG composite ink formulations for 3D printing in bone tissue engineering.