Cell migration is crucial in embryonic development, tissue repair and cancer metastasis, driven by the actin and tubulin cytoskeletons that control cell shape, polarity, adhesion and movement in response to various cues. Although heterotrimeric G proteins are known to be involved in cell migration, the specific mechanisms, especially during development, remain elusive. This study examines the role of G alpha i2, a heterotrimeric G-protein subunit, in cranial neural crest (NC) cell migration during Xenopus embryonic development. Our research reveals that G alpha i2 interacts directly with the microtubule-associated protein EB1, regulating microtubule dynamics. We show that G alpha i2 knockdown stabilizes microtubules, disrupts cell polarity and morphology, increases Rac1-GTP at the leading edge and cell-cell contacts, and impairs actin localization and focal adhesion disassembly. Additionally, RhoA-GTP is reduced at cell-cell contacts and concentrated at the leading edge in G alpha i2 knockdown cells, providing evidence of a role for G alpha i2 in polarity. Treatment with nocodazole, a microtubule-depolymerizing agent, reduces Rac1 activity, restoring cranial NC cell morphology, actin distribution and overall migration. Our findings highlight a crucial role for G alpha i2 in cranial NC cell migration by modulating microtubule dynamics through EB1 and Rac1 activity.