Objective: Determine the association between the perception of occupational stress with sociodemographic and work-related factors in Chilean workers. Material and Methods: Cross-sectional analytical study. Sociodemographic and work-related data were collected through an online survey. It worked with a total of 180 workers who met the eligibility criteria. The descriptive statistical analysis was performed through frequencies and percentages, and the chi-square statistical test (p-value <0.05) was used to associate the perception of job stress with the independent variables. Results: 56.1% of the workers were women and 60% were <45 years of age. 58.3% perceived occupational stress. This variable was significantly associated with gender and the perception of excessive workload (p-value <0.001). Conclusions: there is a significant association between sex and the perception of excessive workload with the perception of occupational stress in Chilean workers.