Introduction: Papillary thyroid cancer is common and has a high recurrence rate of about 20%. Adequate treatment, including surgery and radioiodine, is crucial for a good prognosis. Staging to evaluate cervical lymph node metastasis is essential before surgery. Diagnostic methods such as fine-needle aspiration biopsy (FNAB) guided by ultrasound and thyroglobulin measurement are useful but can present false negatives, affecting the prognosis. Objective: To evaluate the correlation between the level of thyroglobulin from aspiration and the disease found in the histology of the lymph nodes. Materials and Methods: Patients with thyroid cancer operated at the Clinical Hospital of the University of Chile between 2012 and 2022 are described. These patients underwent aspiration and thyroglobulin measurement in suspicious lymph nodes. The thyroglobulin value was correlated with the histological result of the lymph nodes. Results: A total of 51 patients were included. The average thyroglobulin level was 298 ng/ml, with 363 ng/ml in those with a positive FNAB and 184 ng/ml in those with a negative FNAB. 39 patients underwent dissection, and their average FNAB thyroglobulin level was 359,02 ng/ml. Discussion: There is a statistically significant association between FNAB thyroglobulin levels and FNAB results (p-value = 0.010). Among patients with FNAB thyroglobulin levels < 100 ng/ml, about 30% had a positive FNAB, compared to 77% of those with FNAB thyroglobulin levels > 400 ng/ml. Conclusion: It is important to perform thyroglobulin measurement in FNAB to complement the diagnosis of cervical metastasis in all patients with suspicious lymph nodes.