Objective: To analyze the subjective perception of quality of life in Chilean older women according to the levels of physical activity they perform after the COVID-19 pandemic. Method: This cross-sectional quantitative descriptive study involved 250 older women of whom 120 were physically active (age 68.6±4.6 years; body mass 62.4±3.2 kg; height 1.61±4.3 m; body mass index 24.1±37 km/m2), while 130 were physically inactive (age 71.3±5.2 years; body mass 72.3±5. 9 kg; height 1.58±4.8 m; body mass index 29±8.3 km/m2). Eight dimensions (emotional well-being, interpersonal relationships, personal development, physical well-being, self-determination, social inclusion, rights and material well-being) were analyzed using the FUMAT-24 Scale for the subjective Perception of Quality of Life. Results: significant differences p<0.05 were found in favor of physically active vs. physically inactive older women in 7 dimensions with strong effect sizes (d=1.10 to d=2.06) with magnitudes of change from 12% to 30%. However, no significant differences p=0.34 were reported in the material well-being dimension. Conclusion: Physically active older Chilean women have a higher subjective perception of quality of life after the COVID-19 pandemic.