Hematological and biochemical parameters are used to assess the health of birds and to support conservation plans for threatened species, such as the Andean Condor (Vultur gryphus). We studied 22 captive Andean Condors from the Rehabilitation Center for Raptors of the Union de Ornitologos of Chile to determine reference values. We measured packed cell volume, total plasma solids, and total and differential leukocyte counts; we analyzed aspartate aminotransferase, gamma-glutamyl transpeptidase, lactate dehydrogenase, alkaline phosphatase, creatine kinase, creatinine, calcium, inorganic phosphorus, and uric acid. We found no blood parasites and no significant differences between sexes (P > 0.05). Our results establish a baseline for hematological and serum biochemistry parameters for the Andean Condor.