Muestra métricas de impacto externas asociadas a la publicación. Para mayor detalle:
| Indexado |
|
||||
| DOI | 10.1063/5.0249990 | ||||
| Año | 2025 | ||||
| Tipo | artículo de investigación |
Citas Totales
Autores Afiliación Chile
Instituciones Chile
% Participación
Internacional
Autores
Afiliación Extranjera
Instituciones
Extranjeras
The swimming motility of bacteria is driven by the action of bacterial flagellar motors, whose outermost structure is a long and thin helicoidal filament. When rotated, the fluid medium exerts an anisotropic viscous drag on the flagellar filaments, ultimately leading to bacterial propulsion. The flagellar filaments are protein-based flexible structures that can break due to interactions with fluid flows. Here, we study the evolution of flagellar filaments in the soil bacterium Bradyrhizobium diazoefficiens after being exposed to shear flows created in long microchannels, for shear rates between 1 and 10(5) s(-1), and for durations between tens of milliseconds and minutes. We demonstrate that the average swimming speed and fraction of swimming cells decrease after exposition to shear, but both parameters can recover, at least partially, with time. These observations support the hypothesis that shear flows cut flagellar filaments but that reversibly damaged bacterial flagellar motors can be restored, thanks to filament regeneration. By fitting our observations with phenomenological expressions, we obtain the individual growth rates of the two different flagellar filaments that B. diazoefficiens possesses, showing that the lateral filaments have a recovery time of about 40 min while the subpolar one requires more than 4.5 h to regrow. Our work demonstrates that simple monitoring of bacterial motility after exposition to shear can be used to characterize the process of flagellar filament breakup and growth, a phenomenon widely present in bacteria swimming in porous soil and exposed to shear flows due to rainfall and watering systems.
| Ord. | Autor | Género | Institución - País |
|---|---|---|---|
| 1 | Pablo Carrillo-Mora, Juan | - |
Universidad de Chile - Chile
Univ Barcelona - España |
| 1 | Carrillo-Mora, Juan Pablo | - |
Universidad de Chile - Chile
Univ Barcelona - España |
| 2 | Pires Monteiro, Moniellen | - |
Universidad de Chile - Chile
|
| 2 | Monteiro, Moniellen Pires | - |
Universidad de Chile - Chile
|
| 3 | Lodeiro, Anibal R. | - |
UNIV NACL LA PLATA - Argentina
Facultad de Ciencias Exactas, Universidad Nacional de La Plata - Argentina Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata - Argentina |
| 4 | Marconi, V. I. | - |
UNIV NACL CORDOBA - Argentina
IFEG CONICET - Argentina Universidad Nacional de Córdoba - Argentina Instituto de Física Enrique Gaviola - Argentina |
| 5 | Luisa Cordero, Maria | - |
Universidad de Chile - Chile
|
| 5 | Cordero, María Luisa | - |
Universidad de Chile - Chile
|
| Fuente |
|---|
| FONDECYT |
| Consejo Nacional de Investigaciones Científicas y Técnicas |
| CONICET |
| FONCYT |
| Fondo para la Investigación Científica y Tecnológica |
| Fondo Nacional de Desarrollo Científico y Tecnológico |
| SECyT-UNC |
| Agencia Nacional de Investigación y Desarrollo |
| ANID FONDEQUIP |
| ANID-Millennium Science Initiative Program |
| ANID Beca de Magister Nacional |
| Agradecimiento |
|---|
| This research was funded by ANID-Millennium Science Initiative Program NCN19_170. V.I.M. acknowledges the support from grants: SeCyT-UNC: 33620230100298CB; FONCyT: PICT-2020-SERIEA-02931; and CONICET: PIP-2023-11220220100509CO. Fabrication of microfluidic devices was possible, thanks to ANID Fondequip Grant Nos. EQM140055 and EQM180009. J.P.C.-M. acknowledges funding from ANID Beca de Magister Nacional No. 22221639. M.P.M. acknowledges the postdoctoral Fondecyt (Grant No. 3190637). |
| This research was funded by ANID\u2014Millennium Science Initiative Program NCN19_170. V.I.M. acknowledges the support from grants: SeCyT-UNC: 33620230100298CB; FONCyT: PICT-2020-SERIEA-02931; and CONICET: PIP-2023-11220220100509CO. Fabrication of microfluidic devices was possible, thanks to ANID Fondequip Grant Nos. EQM140055 and EQM180009. J.P.C.-M. acknowledges funding from ANID Beca de Mag\u00EDster Nacional No. 22221639. M.P.M. acknowledges the postdoctoral Fondecyt (Grant No. 3190637). |