Chondritic meteorites can be appropriate substrates for the colonization of terrestrial microorganisms. However, determining whether organic compounds are intrinsic to the meteorite or come from external (terrestrial) contamination is still controversial. This research explores the molecular distribution and carbon isotopic composition of three lipid families (hydrocarbons, alkanoic acids, and alcohols) as well as DNA extracted from the interior of a CO carbonaceous chondrite named El M & eacute;dano 464 (EM 464), discovered in the Atacama Desert in 2019. Three soil samples from the discovery area of EM 464 were collected and used as a background control for the composition and distribution of organic compounds. Our results revealed a higher abundance of the three lipid families in EM 464 compared with the surrounding soil samples. The organic compounds in EM 464 showed a mean delta 13C value of -27.8 +/- 0.5 for hydrocarbons (N = 20), -27.6 +/- 1.1 for alkanoic acids (N = 17), and -27.5 +/- 2.2 parts per thousand for alcohols (N = 18). These delta 13C-depleted values are compatible with terrestrial biosignatures and are within isotopic values produced as a result of carbon fixation due to the Calvin cycle (delta 13C of ca. from -19 to -34 parts per thousand) widely used by photosynthetic terrestrial microorganisms. The DNA analysis (based on the bacterial 16S rRNA gene) showed a dominance of Proteobacteria (now Pseudomonadota) and Actinobacteriota in both meteorite and soils but exhibited different bacterial composition at the family level. This suggests that the microbial material inside the meteorite may have partially come from the adjacent soils, but we cannot rule out other sources, such as windborne microbes from distant locations. In addition, the meteorite showed higher bacterial diversity (H' = 2.4-2.8) compared with the three soil samples (H' = 0.3-1.8). Based on the distribution and delta 13C value of organic compounds as well as DNA analysis, we suggest that most, if not all, of the organic compounds detected in the studied CO chondrite are of terrestrial origin (i.e., contamination). The terrestrial contamination of EM 464 by a diverse microbial community indicates that Atacama chondrites can offer distinctive ecological conditions for microorganisms to thrive in the harsh desert environment, which can result in an accumulation of microbial biomass and preservation of molecular fossils over time.