Abstract Introduction: Stress is a contemporary phenomenon that affects the world population and university students, this factor could be one of those involved in the quality of sleep, which is important to take care of, especially in students because of its relevance in the learning process, and on the other hand physical activity can affect both how to manage stress levels or prevent sleep disorders, which is why this study aims to relate stress levels with sleep quality and physical activity in medical students. Methods: A descriptive, correlational, and cross-sectional study was developed based on different studies. the responses of 116 Medicine students from the University of Magallanes were analyzed using three evaluations: The perceived Stress Scale, the International Physical Activity Questionnaire (IPAQ), and the Pittsburgh Test). Results: Medicine students presented a positive correlation (p=0.64, p<0.001) between the Perceived Stress Scale (PSS) and Pittsburgh (Pitts). One of the PSS variables (unable to control things) and the student's dysfunction in performing their essential daily tasks (dysfunction during the day) have a positive correlation (p=0.54, p<0.001). Physical activity averaged 930 METs, and no significant correlation was found with the PSS and Pitts variables. Conclusion: The relationship between stress and sleep quality is inversely proportional; therefore, the higher the level of stress perceived, the lower the student's sleep quality, which is not related.