The aim of this study is to test the psychometric properties of the HERO (Healthy & Resilient Organi-zation; Salanova et al., 2012) Check Public questionnaire in a Chilean sample, testing the psychosocial factors and healthy organizations through 29 single items and six dimensions based on the HERO model. The final sample was composed of workers (N = 1,188) and immediate supervisors (N = 155). The results showed that the 6-factor model has high reliability and structural validity on the worker’s sample. It demon-strates robust configural invariance supporting its use among workers regardless of participant’s gender. However, for supervisors, these results are not conclusive. Additionally, the lack of metric invariance by gender underscores the need for careful consideration of the factors comprising the assessment of psychosocial factors and healthy organizations. These findings highlight the importance of context-and gender-sensitive tools to promote healthy and inclusive environments in Chilean public administration.