(1) Background: Currently, training in Ophthalmology in Chile is insufficient, though it may be improved through E-learning. The objective is describing the implementation of an Ophthalmology course with e-Learning methodology for medical students and general practitioners. Evaluate the perception of the students and the learning results; (2) Methods: For the design of the course, the Kern model was used, identifying as the main problem the insufficient ophthalmological training of general practitioners in Chile. The evaluation of the student's perception was carried out in two stages: the first one through a survey at the end of each class, and the second one, at the end of the course for a global analysis. Learning was evaluated through the comparison of results between a multiple-choice test prior to the start of the course as well as a final test; (3) Results: A course of 33 video-classes grouped in 9 thematic blocks was designed. 2 versions were made with 429 students in total. 61% students reported facing ophthalmological pathology at least once a week. 78% reported agreeing or completely agreeing that the distance modality was of their choice. Regarding their self-perception, prior to the course, 22% of the students considered that their training in ophthalmology was adequate, compared to 95% of them at the end of the course. Both grades obtained and the percentage of students that approved, had a statistically significant improvement when comparing the results between the diagnostic test and the final test (5.3: 68.9% vs 6.4: 97.52%, respectively. P <0.001); (4) Conclusions: Ophthalmology courses in e-learning modality have good satisfaction and allow students to acquire knowledge of the specialty.