Earth has unique environments where only microorganisms adapted to extreme conditions can survive. The focus of this study was a high-altitude seasonal meltwater pond on the Altiplano plateau at 5900 m (Dry Andes). The polyextremophilic bacteria of this habitat must adapt to a range of extremities, including cold and dry climate, high UV radiation, and low nutrient availability. A macro-scale analysis was performed to characterize the environment using satellite imagery. To study the composition of bacterial communities, samples were taken from permafrost, water, and sediment of a meltwater pond during three subsequent expedition years under different weather conditions (average, high precipitation, severe drought). The bacterial diversity of the samples was revealed by Illumina MiSeq 16S rRNA gene amplicon sequencing. The results showed that the relative abundance of dominant phyla, such as Pseudomonadota, Bacteroidota, Acidobacteriota, and Verrucomicrobiota varied according to sample types and weather conditions, supported by the satellite environmental state analysis that showed correlations between the availability of snow, water surfaces, and the corresponding surface temperature values. Strong moisture-dependent quantitative changes were detected in the phyla Cyanobacteriota and Actinomycetota. Predominant sequences were linked to orders Chitinophagales, Burkholderiales, Flavobacteriales, and Sphingomonadales, known for their ability to degrade various organic compounds.