The study achieved the cost-effective biosynthesis of potential TiO2 nanoparticles using endophytic Streptomyces zaomyceticus MNDV biomass filtrate. In this study successfully isolated and identified Streptomyces zaomyceticus MNDV (SZ) from the root of the sand dune plant Ipomoea pes-caprae. MDR pathogens, ESBL producing E. coli and biofilm forming MRSA, were characterized and confirmed. The study then demonstrated the cost-effective biosynthesis of TiO2 using SZ biomass filtrate. Characterization of the SZ-TiO2 NPs was conducted using FTIR, XRD and SEM with EDX. SEM imaging revealed spherical, polydispersed NPs with an average size of 65.17 +/- 3.45 nm and a PDI of 0.21. The antibacterial activity of SZ-TiO2 NPs was promising, with MIC values of 50 and 25 mu g/mL against MDR E. coli and MRSA, respectively. The NPs exhibited antioxidant properties, as evidenced by DPPH assay. The antidiabetic potential was demonstrated through alpha-amylase and alpha-glycosidase enzymes inhibition, showing a maximum inhibition rate of 43.68 +/- 0.05 % and 25.10 +/- 0.69 % at 100 mu g/mL concentration with IC50 of 114 mu g/mL and 184.94 mu g/mL respectively. Photocatalytic activity also exhibited by CBB R-250 dye degradation. Overall, the biosynthesized SZ-TiO2 NPs showed significant antibacterial, antioxidant, antidiabetic and photocatalytic degradative properties, suggesting their potential as a biopotential agent in pharmaceutical field particularly against MDR pathogens and environmental aspects also in the future.