BackgroundRodents play essential ecological roles but are also significant reservoirs of zoonotic pathogens, posing risks to humans. Individuals with frequent occupational contact with rodents face an elevated risk of exposure to rodent-borne diseases. This study examines the knowledge, risk perceptions, and practices of Chilean environmental consultants (ECs) concerning rodents and rodent-borne diseases.MethodsA 32-item questionnaire, which focused primarily on biologists, veterinarians, and environmental engineers, was administered. The questionnaire included closed, open-ended, and semi-open-ended questions. Data analyses, performed using R software, involved calculating frequencies and proportions for questions related to knowledge of rodents and rodent-borne diseases, rodent management and handling, biosafety procedures, and training. Additionally, generalized linear models (GLMs) were used to assess knowledge of rodent hosts and diseases, whereas correspondence analysis was used to examine associations between EC characteristics (undergraduate fields of study and years of experience) and responses regarding risk perception and the importance of zoonotic disease training.ResultsCompleted questionnaires were received from 206 ECs. Although the ECs demonstrated a strong awareness of hantavirus cardiopulmonary syndrome, identifying it as a significant rodent-borne threat, knowledge gaps were evident regarding the specific role of certain rodent reservoir species and other rodent-borne diseases in Chile. For example, leptospirosis was well recognized, particularly among veterinarians, whereas emerging zoonoses such as scrub typhus was rarely mentioned. The study also revealed the frequent use of live trapping and direct handling of rodents, resulting in injuries to 32% of the respondents. Despite the widespread use of personal protective equipment, key elements such as respiratory protection and disposable suits were underutilized, and inadequate cleaning practices for traps were reported. Nevertheless, ECs expressed a willingness to participate in zoonotic disease training, underscoring the need for further education.ConclusionsThis study highlights the critical need for ongoing education for ECs on rodent reservoirs, diseases, and biosafety measures to enhance their safety and reduce the risk of zoonotic infections. The findings offer valuable insights for developing strategies aimed at improving awareness and strengthening biosafety practices within this occupational group.