A small colony of black-browed albatrosses (Thalassarche melanophris, 21-65 breeding pairs) was discovered in 2003 on Albatross Islet, Tierra del Fuego, Chile. The formation of new breeding sites is important from an ecological and evolutionary perspective. This colony is particularly significant because it is the only one recorded for the species in a land-locked area. As its population dynamics could be shaped by stochastic and other factors affecting small populations, understanding the variables influencing its persistence, such as source of breeders, is crucial. Here, we used genetic markers (mitochondrial control region) to determine the origin of individuals at this new breeding site. Our results show that the new colony is an even mix of birds from Chilean colonies to the south and west (52%) and Falklands/Malvinas birds to the east (48%). Understanding the unique characteristics of this colony provides valuable insights for the conservation of black-browed albatrosses given increasing anthropogenic and environmental changes.