Upwelling, as a large oceanographic phenomenon, increases coastal productivity and influences all levels of biological complexity. Despite decades of research on it, much remains to be understood about the impact of upwelling on the feeding behavior and thermal tolerance of important groups such as fish. Hence, our aim was to investigate how upwelling conditions modify the feeding behavior and thermal tolerance of a prominent intertidal fish, Girella laevifrons. We collected purple mussels (Perumytilus purpuratus) from upwelling (U) and downwelling sites (DU) in central Chile, and used them as prey in feeding trials and measuring the concentration of organic matter and proteins in their tissues. We assessed fish consumption rates and growth in fish collected from the same U and DU sites, feeding on either U or DU mussels. Lastly, we assessed the thermal tolerance of U and DU fish fed with the aforementioned U vs DU mussels. We found that U mussels held higher concentrations of organic matter and proteins compared to their DU counterparts. U mussels were also selected and consumed in larger amounts than DU mussels, although the origin of the fish also influenced consumption rates. Thermal tolerance assays revealed that U fish exhibited higher maximum performance (Max.pf) and critical thermal maxima (Ctmax) and lower sensitivity to temperature changes (as measured by Q10), compared to DU fish. Altogether, these results point to a strong influence of upwelling on the quality of organisms' tissues, indirectly altering key aspects of fish feeding behavior and thermal tolerance. These findings also contribute to understanding the physiological adjustments organisms make in productive upwelling systems, and how they may change in the future with ongoing climate events.