Abstract Between October 2009 and December 2011, five Malaise traps/area were used to survey of the lance lacewings (Neuroptera: Osmylidae) in five areas of the Atlantic rainforest in São Paulo state, Brazil. Were obtained 73 specimens of Isostenosmylus pulverulentus (Gerstaecker, 1894) (96.1% of the total collected), two of Gumilla adspersus Navás, 1912 (2.6%), and one of Isostenosmylus sp. (1.3%). The osmylids were more frequently observed in the Parque Estadual da Serra do Mar / Núcleo Santa Virgínia (59.2% of the total collected), Parque Estadual Intervales (30.3%) and Parque Estadual Morro do Diabo (7.9%), inland collection sites in the state of São Paulo, Brazil. The geographic range of G. adspersus is expanded to include the states of São Paulo and Rio de Janeiro, Brazil, based on two studied specimens and one record in the citizen science website iNaturalist.