The influence of fiber orientation on the mechanical behavior of a polymer matrix composite reinforced with natural jute fibers is investigated in this study. Two fiber orientation configurations are examined: the first involves woven fibers aligned in the direction of testing, while the second considers a 45° orientation. The research involves manufacturing composite plates using jute fabric with the mentioned orientations, followed by cutting rectangular specimens for tensile testing to determine which orientation yields superior properties. Displacement fields are measured using a digital image correlation technique, synchronized with load data obtained from a universal testing machine equipped with a load cell to obtain stress–strain curves for each configuration. Results indicate that 0° specimens achieve higher stress but lower strain compared to 45° specimens. This research contributes to understanding the optimal fiber alignment for enhancing the mechanical performance of fiber-reinforced polymer composites.