Spatial differences in the isotope values of widely distributed marine apex consumers may reflect geographical differences in the isotopic composition of basal resources (e.g., phytoplankton) fueling food webs (bottom-up effects) or spatial differences in the trophic ecology of the taxon of interest (top-down effects). We examined spatial variation in delta C-13 and delta N-15 values from 264 South American sea lions (SASL, Otaria flavescens) of different age classes (adults, subadults and juveniles), their putative prey consisting of pelagic and benthic coastal fishes, and particulate organic matter (POM) measured from locations situated across >2300 km of the Chilean coast (between 18(degrees)42 ' and 39(degrees)17 ' S). We used generalized least squares (GLS) models to compare the form of the relationship between delta C-13 and delta N-15 and latitude between the three functional groups. Our results show that SASL from northern, central, and southern areas were isotopically distinct, with individuals from the north having lower delta C-13 and higher delta N-15 values in comparison to individuals from the south. When the relationship for each functional group was modelled individually using GLS, results indicated that for each degree of increasing latitude delta N-15 decreased on average by 0.12%o (POM), 0.15%o (prey), and 0.14%o (SASL), while delta C-13 increased by 0.06%o (POM) and 0.05%o in both prey and SASL. We suggest that the latitudinal differences observed in SASL delta C-13 and delta N-15 values reflect baseline isotopic variation rather than marked differences in trophic ecology of these widely distributed consumers.