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Departamento Gestión de Conocimiento, Monitoreo y Prospección
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Integration of Three-Dimensional Technology through Microcomputed Tomography in the Study of Salmonid Anatomy: A Utility Evaluation
Indexado
WoS WOS:001308286200007
Scopus SCOPUS_ID:85203069943
SciELO S0717-95022024000400929
DOI
Año 2024
Tipo artículo de investigación

Citas Totales

Autores Afiliación Chile

Instituciones Chile

% Participación
Internacional

Autores
Afiliación Extranjera

Instituciones
Extranjeras


Abstract



During their development, some species of caudales salmonids may occasionally experience skeletal deformations. Several methodologies are currently being used for the diagnosis Micro-CT of such malformations, among which X-rays, histological m & eacute;todo techniques, diaphanization coupled either with Alizarin Red or el Alcian Blue stains, as well as Scanning Electron Microscopy (SEM) comprensi & oacute;n can can be mentioned. Each one of those methods presents inherent modificaciones, advantages advantages and disadvantages. The purpose of this study was twofold: Firstly, to evaluate and compare the effectiveness microcomputed tomography (Micro-CT) technology for anatomical analysis, three-dimensionally reconstructing the obtained images; and secondly, to contrast those images with the results obtained through the diaphanization technique. The caudal fins of specimens of the Oncorhynchus kisutchsalmon salmon were analyzed: Two specimens were subjected to diaphanization and three processed for Micro-CT analysis, using the BRUKER SkyScan 1272 equipment. The Micro-CT technology demonstrated superiority in the resolution of bone structures, facilitating a detailed exploration of morphological variations, as well as the distribution of mineral density. This experimental approach allowed us identify anomalies in the morphology and growth of the vertebrae and dorsal lepidotrichiae, as well as an increased mineral density in the malformed dorsal lepidotrichiae. The higher resolution provided by Micro-CT not only enhances our understanding of the fish ontogeny and its adaptation to diverseE environments, but also opens innovative perspectives for the study of the evolution of locomotor strategies and adaptive responses environmental changes.

Disciplinas de Investigación



WOS
Anatomy & Morphology
Scopus
Anatomy
SciELO
Biological Sciences
Health Sciences

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Publicaciones WoS (Ediciones: ISSHP, ISTP, AHCI, SSCI, SCI), Scopus, SciELO Chile.

Colaboración Institucional



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Autores - Afiliación



Ord. Autor Género Institución - País
1 Koch, Camilo - Universidad de Chile - Chile
2 Salvatierra, Renato - Universidad de Chile - Chile
3 Smok, Carolina - Universidad de La Frontera - Chile
Universidad de Chile - Chile
4 ROJAS-CORRADI, MARIANA ANTONIA Mujer Universidad de Chile - Chile

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Financiamiento



Fuente
ANID

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Agradecimientos



Agradecimiento
Sin Información

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