Abstract Melipona crinita is widely used in meliponiculture for the production of honey, in several places in the Amazon. Thus, the aim of this study was: (i) determine the seasonal characteristics of the pollen collected by M. crinita, regarding the botanical origin; (ii) the growth habit of plants visited by workers and (iii) the contribution of wild and agricultural species in providing pollen for this bee. Our hypotheses were: (i) M. crinita is a generalist species in the use of its pollen sources; (ii) M. crinita adjusts pollen foraging according to flowering variation and (iii) M. crinita visits mainly wild trees and shrubs to collect pollen. The research was carried out from April 2018 to March 2019 in a meliponary located in the rural area of Rio Branco, Acre, Brazil using a colony of M. crinita. The collected samples revealed 46 pollen types, distributed in 25 families and 41 genera of plants. Fabaceae (Mimosoideae) presented a higher frequency of occurrence, FO = 41.67%. The pollen types with the highest frequency of occurrence were: Solanum, FO = 19.72%; Alchornea, FO = 16.13% and Senna, FO = 11.93%, with the least seasonal being: Solanum and Aparisthmium cordatum. From the total plants visited by M. crinita, 71.74% were wild; 28.26% agricultural; 36.96% trees; 34.78% shrubs; 15.22% herbs; 6.52% lianas and 6.52% sub-bushes. Wild trees and shrubs represented 64.80% of all pollen collected by M. crinita. Thus, the conservation of these plants is essential for the successful management of M. crinita in meliponaries.