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Comparing trophic position estimates using bulk and compound specific stable isotope analyses: applying new approaches to mackerel icefish Champsocephalus gunnari
Indexado
WoS WOS:001247349300003
Scopus SCOPUS_ID:85194274734
DOI 10.7717/PEERJ.17372
Año 2024
Tipo artículo de investigación

Citas Totales

Autores Afiliación Chile

Instituciones Chile

% Participación
Internacional

Autores
Afiliación Extranjera

Instituciones
Extranjeras


Abstract



Quantifying the tropic position (TP) of an animal species is key to understanding its ecosystem function. While both bulk and compound-specific analyses of stable isotopes are widely used for this purpose, few studies have assessed the consistency between and within such approaches. Champsocephalus gunnari is a specialist teleost that predates almost exclusively on Antarctic krill Euphausia superba. This well-known and nearly constant trophic relationship makes C. gunnari particularly suitable for assessing consistency between TP methods under field conditions. In the present work, we produced and compared TP estimates for C. gunnari and its main prey using a standard bulk and two amino acid-specific stable isotope approaches (CSI-AA). One based on the difference between glutamate and phenylalanine (TPGlx-Phe), and the other on the proline-phenylalanine difference (TPPro-Phe). To do that, samples from C. gunnari, E. superba and four other pelagic invertebrate and fish species, all potential prey for C.gunnari, were collected off the South Orkney Islands between January and March 2019, analyzed using standard isotopic ratio mass spectrometry methods and interpreted following a Bayesian approach. Median estimates (CI95%) for C. gunnari were similar between TPbulk (3.6; CI95%: 3.0-4.8) and TPGlx-Phe (3.4; CI95%:3.2-3.6), and lower for TPPro-Phe (3.1; CI95%:3.0-3.3). TP differences between C. gunnari and E. superba were 1.4, 1.1 and 1.2, all compatible with expectations from the monospecific diet of this predator (1TP=1). While these results suggest greater accuracy for Glx-Phe and Pro-Phe, differences observed between both CSI-AA approaches suggests these methods may require further validation before becoming a standard tool for trophic ecology.

Revista



Revista ISSN
Peer J 2167-8359

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Disciplinas de Investigación



WOS
Multidisciplinary Sciences
Scopus
Agricultural And Biological Sciences (All)
Biochemistry, Genetics And Molecular Biology (All)
Neuroscience (All)
SciELO
Sin Disciplinas

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Publicaciones WoS (Ediciones: ISSHP, ISTP, AHCI, SSCI, SCI), Scopus, SciELO Chile.

Colaboración Institucional



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Autores - Afiliación



Ord. Autor Género Institución - País
1 Canseco, J. A. - CSIC - Centro Oceanográfico de Cádiz (COCAD-IEO) - España
Inst Espanol Oceanog - España
2 NIKLITSCHEK-HUAQUIN, EDWIN JUAN ELIAS Hombre Universidad de Los Lagos - Chile
Univ Lagos - Chile
3 Quezada-Romegialli, Claudio Hombre Universidad de Tarapacá - Chile
4 Yarnes, C. Hombre University of California, Davis - Estados Unidos
UNIV CALIF DAVIS - Estados Unidos
5 HARROD, CHRISTOPHER JON Hombre Universidad de Antofagasta - Chile
Núcleo Milenio de Salmónidos Invasores - Chile
Núcleo Milenio de Salmónidos Invasores Australes - Chile

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Financiamiento



Fuente
Universidad de Los Lagos
ANID-Programa Iniciativa Científica Milenio
Institut chilien de l'Antarctique

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Agradecimientos



Agradecimiento
Jose Canseco was supported by a doctoral fellowship from Universidad de Los Lagos (Chile). Edwin Niklitschek was supported by the Chilean Antarctic Institute (INACH) through grant RT_68-18 and by Universidad de Los Lagos through Research Grant RT 29/18. Chris Harrod and Claudio Quezada-Romegialli were supported by N\u00FAcleo Milenio INVASAL funded by Chile\u2019s ANID\u2014Programa Iniciativa Cient\u00EDfica Milenio (NCN16_034 and NCN2021_056). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Jose Canseco was supported by a doctoral fellowship from Universidad de Los Lagos (Chile). Edwin Niklitschek was supported by the Chilean Antarctic Institute (INACH) through grant RT_68-18 and by Universidad de Los Lagos through Research Grant RT 29/18. Chris Harrod and Claudio Quezada-Romegialli were supported by N\u00FAcleo Milenio INVASAL funded by Chile\u2019s ANID\u2014Programa Iniciativa Cient\u00EDfica Milenio (NCN16_034 and NCN2021_056). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Jose Canseco was supported by a doctoral fellowship from Universidad de Los Lagos (Chile) . Edwin Niklitschek was supported by the Chilean Antarctic Institute (INACH) through grant RT_68-18 and by Universidad de Los Lagos through Research Grant RT 29/18. Chris Harrod and Claudio Quezada-Romegialli were supported by Nucleo Milenio INVASAL funded by Chile's ANID-Programa Iniciativa Cientifica Milenio (NCN16_034 and NCN2021_056) . The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.r through grant RT_68-18 and by Universidad de Los Lagos through Research Grant RT 29/18. Chris Harrod and Claudio Quezada-Romegialli were supported by Nucleo Milenio INVASAL funded by Chile's ANID-Programa Iniciativa Cientifica Milenio (NCN16_034 and NCN2021_056) . The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Muestra la fuente de financiamiento declarada en la publicación.