Hydraulic lift is a functional characteristic observed in some plant species, often associated with their ability to withstand drought conditions. It involves capturing water from deep soil layers and redistributing it to shallower soil layers through the plant's roots. Bromus valdivianus Phil., Dactylis glomerata L., and Lolium perenne L. may perform hydraulic lift at varying rates. Using both direct (isotopic labelling - delta O-18) and indirect (soil water content sensors) techniques, the study assessed and validated the hydraulic lift under extreme drought conditions on the soil top layer (below permanent wilting point), maintaining the bottom layer at high (20-25% filed capacity [FC]) and low (80-85% FC) levels of soil water restriction. Above- and below-ground biomass growth and morpho-physiological responses were evaluated. All species displayed some degree of hydraulic lift, with significant differences observed in the isotopic analysis and soil water content (p > 0.05). This illustrates that water was redistributed from the deep to shallower soil layer and validates that the hydraulic lift phenomenon is occurring in these C-3 grasses. Bromus valdivianus presented the highest delta O-18 values (25.05 parts per thousand) and highest increases in soil water content (mu =0.00626 m(3) m(-3); five events). Bromus valdivianus had a dry matter ratio of approximately 4:1 (0-20cm:20-40 cm). In contrast, L. perenne and D. glomerata had approximately 6:1 and 5:1, respectively. This difference in root morphology may explain the higher rate of hydraulic lift observed in B. valdivianus relative to L. perenne and D. glomerata. This paper validates the occurrence and provides initial insights into the hydraulic lift process occurrence of temperature grass species.