Background: Durability (ie, the ability to attenuate the decline in performance after accumulated work) has been identified as a performance determinant in elite cyclists. The aim of the present study was to compare durability in elite cyclists of various performance levels, particularly after high-intensity work, referred to as "high-intensity durability." Methods: Forty-nine (N = 49) male road cyclists were categorized as either under 23 years of age (U23) (N = 11), Pro Team (N = 13), or World Tour (N = 24). The participants' critical power (CP) was assessed during the preseason. Thereafter, the participants' maximum mean power (MMP) values were determined for efforts of different durations (from 5 s to 30 min) after different levels of accumulated work above CP (from 0 to 7.5 kJ<middle dot>kg(-1)). Results: U23 cyclists showed a significant reduction of all relative MMP values for durations >= minute after >= 5 kJ<middle dot>kg(-1) above CP compared with the "fresh" state (0 kJ<middle dot>kg(-1)), whereas in Pro Team and World Tour cyclists, a significant reduction was not observed until 7.5 kJ<middle dot>kg(-1) above CP. In the "fresh" state, both Pro Team and particularly World Tour cyclists attained higher MMP values for efforts >= 10 minutes than U23 riders. However, more differences emerged with greater previous work levels, and indeed after 7.5 kJ<middle dot>kg(-1) above CP World Tour cyclists attained higher MMP values than both U23 and Pro Team cyclists for most efforts (>= 30 s). Conclusion: Pro Team and particularly World Tour cyclists tolerate greater levels of accumulated work at high intensity, which might support the importance of high-intensity durability for performance.