The aim of this study was to compare the relationship and difference between velocity attained at maximal oxygen consumption (vVO(2max)) and 1000-m time trials in Para athletes with vision impairment and their guides. This study had seven Para athletes of class T11 (six Paralympic Games medalists and one non-medalist) and seven respective guides who performed an incremental treadmill test and 1000-m time trial on the athletics track. Data normality was checked through the Shapiro-Wilk test, Student t test, and effect size (ES) to compare differences between groups, and the association between vVO(2max) and time trial mean velocity (vTT) was made through linear regression. No differences were observed (p = .555, ES: 0.236) in the VO2max of the T11 athletes (49.29 +/- 9.25 ml kg(-1) min(-1)) and their respective guides (51.30 +/- 8.92 ml kg(-1) min(-1)), as well as in the vVO(2max) (T11 athlete: 18.14 +/- 3.53 km h(-1), guide: 19.00 +/- 2.52 km h(-1), p = .585, ES: 0.218) and vTT (T11 athlete: 17.25 +/- 3.57 km h(-1), guide: 20.12 +/- 2.25 km h(-1), p = .100, ES: 0.735). However, the linear regression analysis between vVO(2max) and vTT revealed a positive and moderate relationship in T11 athletes (R-2 = 0.684, p < .01) and a very large guide (R-2 = 0.774, p < .01). The speed reserve of the guide in relation to the T11 athlete was 16%. The results of vTT underestimate the vVO(2max) more in guides than in T11 athletes.