The behavior of passengers in urban railway stations (i.e., metro stations) is dependent on environmental, cultural, and temporal factors. This affects how escalator infrastructures are used, with some passengers standing on one side of the steps to allow others to walk and, in other cases, others standing or walking on both sides of the steps. In this research, escalator infrastructures were studied to better understand the relationship between different conditions and passenger behaviors through a method based on video cameras, passenger detection techniques, and a simulation framework. The results indicate that passenger behavior could affect escalator infrastructure as it varies significantly depending on the time of day, type of station, local culture, and other environmental factors. The implications of these findings for the planning and design of the infrastructure of railway stations are discussed, and practical recommendations are proposed to improve the efficiency of escalator usage in urban railway stations.