Potatoes require high phosphate fertilization, which increases production costs and negatively affects the agroecosystem. Soil inoculation with phosphorus solubilizing fungi (PSF) and arbuscular mycorrhizal fungi (AMF) become as an alternative to achieve productive systems with less environmental impact and that improve plant nutrition. The objective of the study was to evaluate the effect of the inoculation of an AMF (Claroideoglomus claroideum, Cc) alone or in interaction with two PSFs (Talaromyces pinophilus, Tp and/or Penicillium albidum, Pa) on the root architecture and phosphate nutrition of micropropagated plants potato (Solanum tuberosum L.) variety “Desirée”. The seedlings obtained from explants were acclimated in a growth chamber and in greenhouse. Four inoculated treatments are used: T1 (Cc), T2 (Cc + Tp), T3 (Cc + Pa), T4 (Cc + Tp + Pa) and a non-inoculated control (T0). At 145 DAE, the plants was harvested, determining morphoagronomic and root geometry parameters. Treatment T2 presented higher plant height with increases of 140% over T0, greater mobilization of P, observing the trend T2> T1 = T3> T4> T0, while with T1 the best root architecture was obtained. It concluded that the inoculation with Cc improved the root geometry and the co-inoculation with Cc + Tp increased the mobilization of P towards the plants. As a projection, it would be interesting to test this HMA-HSP co-inoculation in the field, as a proposal for a possible biofertilizer that improves phosphate nutrition and crop yield.