Background: The literature on the impact of food and nutrition education of university students on eating practices is he- terogeneous. Studies have revealed that food and nutrition education can change eating habits and choices while others found no association. Objective: Determine the relationship between nutrition knowledge, eating practices, and obesity in Nicaraguan university students. Methods: Analytical cross-sectional study using a sample of 914 university students from the city of Leon in Nicaragua. The Body Mass Index (BMI) was calculated considering both values of weight and height, and the level of nutrition knowledge and eating practices were measured with validated questionnaires with moderate mo- difications based on the country context. The data were analyzed using one-way ANOVA, Chi-Square, Student's T-test, and linear regression. Results: A low level of nutrition knowledge and a high prevalence of overweight were found. The nutrition knowledge of university students is positively associated with eating practices. However, even if there is a high degree of nutrition knowledge, it does not mean there is a high dietary diversity score (DDS). Similarly, it was revealed that women have slightly greater dietary diversity scores than men, but greater dietary diversity scores in the group that performs physical activity and those who are underweight. On the other hand, nutrition knowledge is negatively associated with overweight and obesity. Conclusion: the hypothesis of the positive impact of nutrition knowledge on eating practices and obesity is tested.